November 2017 Homogenizer & Homogenizing News
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Homogenizing tip of the day
PRO Generator Probes are precision crafted
PRO Generators are unique in their design and this is what really sets us apart from the competition. The double bearing design of our probes provides fast & complete sample breakdown with precision & consistency.
Also, PRO Generator Probes are heat resistant to 390°F; all probe components can be autoclaved!
Homogenizing Research
In the news: Biofuel Research
The Smithsonian Magazine (Barth, 2017) wrote about research that was being done on abundant species that could be used as biofuel. PRO Scientific is happy to have been involved in this research and we have worked with customers homogenizing turfgrass.
PRO Scientific high shear homogenizers provide aggressive homogenization to effectively break down the various agricultural waste products such as cornstalks, poplar trees, and switchgrass.
PRO Scientific explains the field of homogenizing
The field of homogenizing encompasses a very broad area and is constantly evolving.
The word homogenize means "to make or render homogeneous" while homogeneous means "having the same composition, structure or character throughout". Homogenizing is what is called an umbrella word - a word which covers a very large area. When someone says that they are homogenizing, they may mean that they are actually doing one or more of the following: blending, mixing, disrupting, emulsifying, dispersing, stirring etc.
The current processes or methods of homogenizing can be broken down into three (3) major categories: mechanical, ultrasonic and pressure.
Mechanical homogenizers can be broken down into three (3) separate categories: rotor-stator homogenizers, blade type homogenizers, and bead homogenizers.
Learn more about the full range of Rotor-Stator Homogenizers available from PRO Scientific; including Package Kits, Hand-Held or Stand Mounted, Benchtop and Programmable Benchtop Homogenizers.
Fall Homogenizing
PRO homogenizer used to process cucurbita (pumpkin) pollen. (C. Carrizo García, 2014)
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Instituto Multidisciplinario de Biología Vegetal (CONICET), Córdoba, Argentina
https://proscientific.com/rotor-stator-homogenizers/
Seeds, pulp and pumpkin chunks homogenized with the SB-1, PRO250 and Multi-Prep Homogenizers
Food and Agricultural Research
PRO Scientific Homogenizers are ideal bench scale high-shear homogenizers for both the food and agricultural laboratories. PRO Generators are precision crafted with a very narrow clearance between the inner rotating shaft and outer tube/chamber. This allows for quick, effective and repetitive processing, meaning you will get the results you want each and every time. Specialty HD or Titanium Generators are also available to address concerns of trace analysis. This allows for quick, effective and repetitive processing
Shear numbers are known for each and every PRO Quick Connect Generator. This means we can easily work with customers when the times comes to scale up from our high shear homogenizer equipment to larger industrial homogenizers.
A range of powerful PRO high shear Homogenizers are available to handle a wide range of viscosities and volumes.
PRO250 Homogenizer - 3/4 hp, 576 Watt
PRO25D Digital Homogenizer - 3/4 hp, 576 Watt
PRO400D or 400PC Homogenizer - 1 3/4 hp, 1305 Watt
A PRO Homogenizer was used to emulsify anhydrous milk fat with various surfactants as part of food science research.
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Bio-Gen PRO200 homogenizer produced emulsions w/lactoferrin-based nanoparticles.
(G. Shimoni, 2013)
Homogenizer Reviews
Our customer at WUSTL has been using a PRO homogenizer for years now to process their mouse skin tissue. They recently gave our PRO Homogenizers a 5-star review for ease of use, after sales services and value for money!
Reviews are collected by a 3rd party company, SelectScience
October 2017 Homogenizer & Homogenizing News
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Homogenizing tip of the day
Faster isn’t always better when it comes to homogenizing
Always begin homogenizing at a low rpm, and then gradually increase the speed to your target level. Running too fast can cause two things to happen:
A quick increase in speed will push the sample away from the probe and inhibit homogenization.
If you are processing a small volume of sample and running at max speed be careful that you aren't pumping the sample through the probe quicker than it can recover. If the sample is moving through the probe quicker than it can recover, the probe will begin to homogenize pockets of air. These moments of dry homogenizing can damage the probe.
When using Multi-Gen 7XL probes, it is not recommended to run at more than half speed (16,000 RPM). If this is required, please contact a PRO Scientific representative to discuss modification of your equipment.
Review more homogenizing tips online.
Tissue Tearor, Disperser or Homogenizer?
What’s the difference and what do you need for your application?
The field of homogenizing encompasses a very broad area and is constantly evolving.
The word homogenize means "to make or render homogeneous" while homogeneous means "having the same composition, structure or character throughout". Homogenizing is what is called an umbrella word - a word which covers a very large area. When someone says that they are homogenizing, they may mean that they are doing one or more of the following, blending, mixing, disrupting, emulsifying, dispersing, stirring etc. Therefore, when the word homogenizing is used it may mean any one or more of the above-mentioned processes.
The current processes or methods of homogenizing can be broken down into three (3) major categories: mechanical, ultrasonic and pressure.
These are reviewed in detail online.
The key to good homogenizing
When it comes to homogenizing, the key to homogenizing is the generator probe as this is the component that actually comes into contact with your sample.
Whether you are equipping your PRO Homogenizer with one of our PRO Quick Connect Generator Probes or our Multi-Gen 7XL Multi-Sample
Generator Probes to homogenize your tissue samples, you will get consistent results each and every time.
All PRO Generator Probes are precision crafted to ensure efficient and effective homogenization and are manufactured out of 316SS to be chemically compatible with your sample and reagents.
Multi-Sample Homogenizing
Multiple sample homogenizing can be time-consuming and can create concerns of cross-contamination. PRO Scientific offers the solution.
Multi-Gen 7XL compared to plastic disposable probes
You may be concerned about cross-contamination between samples while homogenizing, but remember…plastic doesn’t cut it. Multi-Gen 7XL Stainless Steel Probes are the better alternative to plastic disposable probes or tips.
PRO Scientific Multi-Gen 7XL features several advantages over plastic disposable probes:
Higher sample breakdown yields
Multi-Gen probes provide higher sample breakdown yields over plastic probes. This is true even for the homogenization of difficult samples such as tough tissues. The clearance between the stainless-steel rotor-stator is extremely tight and along with its open-slotted teeth, this leads to higher shear rates. This provides faster overall cutting ability and better performance in less time compared to plastic probes.
Extended usage
Multi-Gen Probes were quality tested and run for 11 hours and showed no signs of wear because of its quality design and superior material. Plastic versions have limited usage, depending on brand, and some will even only allow 1-2 uses. Plastic probes are commonly recommended for single use. In some cases, an overused plastic disposable probe or tough sample can shatter or splinter during homogenizing.
Chemical compatibility
Multi-Gen Probes, manufactured out of 316 Stainless Steel and PTFE, provide a greater reagent compatibility compared to plastic models, which have significant limitations. Cleaning Multi-Gen probes is easier because of this construction, and probes can be continually sterilized by any method, including flaming and autoclaving. Plastic versions can only be cleaned with certain methods, and depending on brand, may be autoclaved 1-2 times no matter the sample.
Environmentally & budget conscious
Multi-Gen Probes are environmentally and budget friendly compared to continually discarding plastic versions. Avoid making your homogenizing a consumable. Multi-Gen Probes have the ability to be continually used, unlike a plastic probe that will need to be purchased again and again.
Learn more why the Multi-Gen 7XL probes are the better alternative to plastic probes or tips.
Homogenizer Applications
PRO Multi-Gen 7XL, multi-sample homogenizing of tissue samples for RNA extraction.
University of Florida & UF Genetics Institute and Graduate Program 
https://proscientific.com/multi-sample-homogenizer-kit/
A Comparative Analysis of Gene Expression Profiles during Skin Regeneration in Mus and Acomys PLOS ONE, Vol. 10, No. 11. (25 November 2015), e0142931, doi:10.1371/journal.pone.0142931 by Jason O. Brant, Maria-Cecilia Lopez, Henry V. Baker, W. Brad Barbazuk, Malcolm Maden
Bio-Gen PRO200 used for RNA extraction of conjunctival epithelial cells.
Shanghai Jiao Tong University, Dept of Ophthalmology
https://proscientific.com/hand-held-homogenizers/bio-gen-pro200-homogenizer/
Yao, Q., Zhang, W., Hu, Y., Chen, J., Shao, C., Fan, X., Fu, Y."Electrospun collagen/poly(L-lactic acid‑co‑ε‑caprolactone) scaffolds for conjunctival tissue engineering". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4141-4147. https://doi.org/10.3892/etm.2017.5073
Homogenizer Reviews
PRO Multi-Gen 7XL
Another fantastic review of the Multi-Gen 7XL Homogenizing package. Courtesy of Tulane University and 3rd part company, SelectScience
Mechanically and ultrasonically homogenizing your samples?
See how the DPS-20 Two-Step Homogenizing system can help.
Resources
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