Homogenizer Tips

Faster isn't always better.

Always begin homogenizing at a low rpm, and then gradually increase the speed to your target level. Running too fast can cause two things to happen: 

A quick increase in speed will actually push the sample away from the probe and inhibit homogenization.
If you are processing a small volume of sample and running at max speed be careful that you aren't pumping the sample through the probe quicker than it can recover.  If the samples are moving through the probe quicker than it can recover the probe will begin in essence to homogenize pockets of air and this dry homogenizing can damage the probe.  

When using Multi-Gen 7XL probes, it is not recommended to run at more than half speed (16,000 RPM).  If this is required, please contact a PRO Scientific representative to discuss the modification of your equipment. 

Make sure you are processing the recommended sample volume for the generator/generator assembly you are using. 

Sample Size 

Generators are capable of homogenizing a sample size from 0.03ml to 20L.
(Larger non-viscous samples may be homogenized with deflector head attachment)

• 5mm or 7mm generators can fit into 1.5ml - 2ml eppendorf tubes.
• With a 7mm generator, the generator probe won’t touch the bottom of conical microtubes, but substance may get pulled up into generator. The generator will touch the bottom of a flat bottom microtube.
• The sample, if solid, should be no longer than half the diameter of the generator.

Generator Width 

Generally, you want to homogenize and use a generator as wide as the tube you are using.

• This will allow for faster and better homogenizing because no material will "escape" from the generator.
• Keep in mind that there is some displacement of material once the generator enters the tube or container.

Location of the Generator Within the Sample 

Place the generator off-center in the tube or container for the best results.

• This will prevent material from getting "stuck" on the walls of the tube and not being homogenized.
• Place the generator 1/3 of the sample height off of the bottom of the tube or container.

Configuration of Container& Generator Length

When using a generator 120mm or greater in length, it is important to remember that these generators have a mid-bearing. This “bearing” helps prevent bending or movement of the rotor within the tube.

This mid-bearing still needs to be kept moist during homogenizing and therefore should be kept covered by the substance being homogenized, not doing this will burn out the bearing, produce excessive amounts of heat and damage your generator. This will also cause the sample to become dingy and blackened.

If using too long of a generator for your sample size, you may start to pump air because the generator cannot recover fast enough.

Use as short a generator you can for your container/tube.