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What I'm Homogenizing A-K

Acetate
PRODUCT APPLICATION REPORT
MATERIAL: Acetate fiber
VOLUME OF MATERIAL: 1 g
MEDIUM: Methanol and Freon solvent
VOLUME OF MEDIUM: 100 ml
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Oester blender
EQUIPMENT USED: PRO250 Homogenizer with stand or PR0300 D Homogenizer with safety sealed generators
APPLICATION AND RESULTS: Obtained needed effect in 10 seconds, 1/6 the time of the blender.

Aribidopsis seedlings
PRODUCT APPLICATION REPORT
MATERIAL: 5 ml
VOLUME OF MATERIAL: Aribidopsis seedlings
MEDIUM: Extraction buffer
VOLUME OF MEDIUM: 150 ml
PROCESS: RNA extraction
PREVIOUS OR CURRENT METHOD: Brinkmannn polytron
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: Excellent homogenization of entire seedling after grinding with mortar and pestle. Got large yields from the PRO200 Homogenizer  and acceptable RNA samples.

Aspirin tablets (20 tablets)
PRODUCT APPLICATION REPORT
MATERIAL: Aspirin tablets (20 tablets)
VOLUME OF MATERIAL: 325mg per tablet
MEDIUM: None
VOLUME OF MEDIUM: None
PROCESS: Homogenize to a fine powder
PREVIOUS OR CURRENT METHOD: Unknown
EQUIPMENT USED: cra:
Test 1 & 2: 30mm generator (02-30150)
Test 3 & 4: 20mm generator (02-20150)
APPLICATION AND RESULTS: Various tests were run as follows:

Test 1: 10,000 rpm - 1 minute moving up, down, and around in a glass jar.

Test 2: 10,000 rpm - 45 seconds moving up, down, and around in a 150m1 glass beaker.

Test 3: 30,000 rpm - 1 minute moving up, down, and around in a 150ml glass beaker.
Test 4: 30,000 rpm - 35 seconds moving up and down in a 50ml conical tube.

RECOMMENDATIONS: 
Test 1: fine powder with no lumps. 
Test 2: fine powder with no lumps. 
Test 3: a powder with some lumps. 
Test 4: very fine powder with no lumps. In all four tests, the aspirin was reduced to a powder.

Bacteria
PRODUCT APPLICATION REPORT
MATERIAL: Bacteria
VOLUME OF MATERIAL: 500ml
MEDIUM: Culture
VOLUME OF MEDIUM: Not reported
PROCESS: Complete dispersion with low foaming
PREVIOUS OR CURRENT METHOD: Brinkmannn Polytron
EQUIPMENT USED: PRO200 Homogenizer with 20mm PRO Safety Sealed Chamber Assembly
APPLICATION AND RESULTS: Worked perfectly with minimal foaming.

Bacterial phase-filaments
PRODUCT APPLICATION REPORT
MATERIAL: Bacterial phage-filaments
VOLUME OF MATERIAL: .015-.02 ml
MEDIUM: Aqueous buffer
VOLUME OF MEDIUM: 1-1.5 ml
PROCESS: disruption of the filament
PREVIOUS OR CURRENT METHOD: Mechanical Shear and Hand-press
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: Achieved desired results in 25-30 sec.

Biological tissue
PRODUCT APPLICATION REPORT
MATERIAL: Biological tissue
VOLUME OF MATERIAL: 5ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Break down tissue for analysis and testing
PREVIOUS OR CURRENT METHOD: Hand mixer
EQUIPMENT USED: PRO200 Homogenizer with 7mm generator
APPLICATION AND RESULTS: Achieved desired results

Bone and joint tissue
PRODUCT APPLICATION REPORT
MATERIAL: Bone and joint tissue
VOLUME OF MATERIAL: Micro volumes
MEDIUM: Tween-80 in TSB
VOLUME OF MEDIUM: Micro volumes
PROCESS: Bacteria in the bone and joint tissue were isolated
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer
APPLICATION AND RESULTS: Achieved desired results for overnight culture

Bovine brain
PRODUCT APPLICATION REPORT
MATERIAL: Bovine brain
VOLUME OF MATERIAL: 50 g (10mm gen.) and 150 g (37mm gen.)
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Brinkmannn Polytron PT10 / 35
EQUIPMENT USED: PRO250 Homogenizer with stand or PR0300 D Homogenizer with 10 & 30mm generators
APPLICATION AND RESULTS: Successfully homogenized the Bovine brain
with both volumes and generators.

Bovine neutrophil pellets
PRODUCT APPLICATION REPORT
MATERIAL: Bovine neutrophil pellets
VOLUME OF MATERIAL: Not reported
MEDIUM: TRIzol
VOLUME OF MEDIUM: 1 ml
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer
APPLICATION AND RESULTS:
DOI:		 Achieved desired homogenization results,
stored at -80°C for RNA extraction
Application report

Bovine thyroid tissue
PRODUCT APPLICATION REPORT
MATERIAL: Bovine thyroid tissue
VOLUME OF MATERIAL: 50ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: Worked better than expected.

Bovine tongue-epithelial tissue
PRODUCT APPLICATION REPORT
MATERIAL: Bovine tongue-epithelial tumor tissue
VOLUME OF MATERIAL: cut into small pieces
MEDIUM: Not reported
VOLUME OF MEDIUM:
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer
APPLICATION AND RESULTS:
DOI:		 Achieved desired homogenization results 
http://dx.doi.org/10.3892/mmr.2014.2887
Cat Fish
PRODUCT APPLICATION REPORT
MATERIAL: Cat Fish
VOLUME OF MATERIAL: Not reported
MEDIUM: None used
VOLUME OF MEDIUM: None used
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO250 Homogenizer with stand or PR0300 D Homogenizer with 473 ml gladd chamber assembly & 2" diameter blade 
APPLICATION AND RESULTS:

Successfully homogenized the Catfish.
However, some skin did get wrapped around the blade.Equipment supplied was demo equipment and the
blades may not have been as sharp as a production blade. This could have contributed to the skin wrapping around the blade.
Recommend using a sharpened blade. The entire length of the blade should be sharpened, instead of the standard tips of the blade.

Cell membrane
PRODUCT APPLICATION REPORT
MATERIAL: Cell membrane
VOLUME OF MATERIAL: 50 ml
MEDIUM: PBS type
VOLUME OF MEDIUM: 50 ml
PROCESS: Cell membrane preparation
PREVIOUS OR CURRENT METHOD: Large homogenizers
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: Quicker and easier than other homogenizers along with achieving same results.

Cells
PRODUCT APPLICATION REPORT
MATERIAL: Cells
VOLUME OF MATERIAL: .5 ml
MEDIUM:  Not reported
VOLUME OF MEDIUM: 5 ml
PROCESS: Break open cell membranes
PREVIOUS OR CURRENT METHOD: Freezing and thawing
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: Completely homogenized and broken down in
fifteen seconds.

Chicken cartilage
PRODUCT APPLICATION REPORT
MATERIAL: Chicken cartilage
VOLUME OF MATERIAL: Not reported
MEDIUM: Sucrose
VOLUME OF MEDIUM: 1/2 ml
PROCESS: Homogenization without destroying the sub-cellular
components
PREVIOUS OR CURRENT METHOD: Dounce homogenizer
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: Ran well and kept sub-cellular components

Chicken gizzards
PRODUCT APPLICATION REPORT
MATERIAL: Chicken gizzards
VOLUME OF MATERIAL: 50g
MEDIUM: Acetone
VOLUME OF MEDIUM: 200 ml
PROCESS: Homogenization for chemical analysis
PREVIOUS OR CURRENT METHOD: Polytron, Tissuemizer
EQUIPMENT USED: PRO250 Homogenizer w/stand or PRO300 Homogenizer with 20mm Generator or Blade PRO Safety Sealed Chamber Assembly
APPLICATION AND RESULTS: Blade system did not completely
homogenize the sample, but results were adequate enough. However,
the 20mm generator completely homogenized the sample in two
minutes after pre-chopping the gizzard into 1 cm pieces.
RECOMMENDATIONS: Cutting large chunks of tissue into pieces
will allow a greater number of applications with the
generators.

Chicken tissue
PRODUCT APPLICATION REPORT
MATERIAL: Chicken tissue
VOLUME OF MATERIAL: 30 mg of tissue
MEDIUM: RLT buffer
VOLUME OF MEDIUM: 600 ul
PROCESS: 30s homogenization twice
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer with MULTI-GEN 7 generator
APPLICATION AND RESULTS: Successfully homogenized for RNA processing
Link DOI: http://dx.doi.org/10.1016/j.cimid.2012.02.004

Chlorophilia
PRODUCT APPLICATION REPORT
MATERIAL: Chlorophilia
VOLUME OF MATERIAL: 5-10 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Water quality testing
PREVIOUS OR CURRENT METHOD: Ultrasonic
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: Worked in homogenizing the sample

Citrus bark
PRODUCT APPLICATION REPORT
MATERIAL: Citrus bark
VOLUME OF MATERIAL: 5 ml
MEDIUM: Phosphate buffer
VOLUME OF MEDIUM: Not reported
PROCESS: Extraction
PREVIOUS OR CURRENT METHOD: Liquid nitrogen and break up with hammer
EQUIPMENT USED: PRO200 Homogenizer with 7mm or 10mm generator
APPLICATION AND RESULTS: Able to achieve desired results with both
generator sizes.

Clumped white cell agluts
PRODUCT APPLICATION REPORT
MATERIAL: Clumped white cell agluts
VOLUME OF MATERIAL: Not reported
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Steinbach homogenizer
EQUIPMENT USED: PRO200 Homogenizer with 7mm or 10mm generator
APPLICATION AND RESULTS: Worked well with both
generator sizes.

Corpus luteum
PRODUCT APPLICATION REPORT
MATERIAL: Corpus luteum
VOLUME OF MATERIAL: Not reported
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization for RNA extraction and RT
PREVIOUS OR CURRENT METHOD:
EQUIPMENT USED: Bio-Gen PRO200 Homogenizer
APPLICATION AND RESULTS:
DOI:

 The CLs were homogenized with TRIzol Reagent 

http://dx/doi.org/10.1530/REP-13-0398

Cotton roots
PRODUCT APPLICATION REPORT
MATERIAL: Cotton roots
VOLUME OF MATERIAL: 5-6 ml
MEDIUM: SPW
VOLUME OF MEDIUM: 5-6 ml
PROCESS: Reduce in size and release hormones
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: Worked well with tender roots, but not as
effective with hardened stems. For best results use a tube a
little larger in diameter than the generator

Cranberries
PRODUCT APPLICATION REPORT
MATERIAL: Cranberries
VOLUME OF MATERIAL: 30 ml
MEDIUM: Water
VOLUME OF MEDIUM: 20 ml
PROCESS: Homogenization of berries in robot assembly line
PREVIOUS OR CURRENT METHOD: None
EQUIPMENT USED: PRO250 Homogenizer with stand or PR0300 D Homogenizer with gblade and chamber assembly
APPLICATION AND RESULTS: Worked well

Cultured bone marrow macrophagecells
PRODUCT APPLICATION REPORT
MATERIAL: Cultured bone marrow macrophage cells
VOLUME OF MATERIAL: Not reported
MEDIUM: Phosphate buffered saline
VOLUME OF MEDIUM: 2.25 ml
PROCESS: Membrane suspension from whole cell suspension
PREVIOUS OR CURRENT METHOD: Ultrasound, barrel and pestle
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: With old system, only able to achieve 400
rpms of binding above the blank value of 100. With the PRO200 Homogenizer 
system, achieved 4000 rpms within seconds. However, results were
too good for the experiment that was being conducted.

Drosophilia aelanogaster
PRODUCT APPLICATION REPORT
MATERIAL: Drosophilia melanogaster
VOLUME OF MATERIAL: 3-10 fruit flies
MEDIUM: Aqueous buffer
VOLUME OF MEDIUM: 100-300 ml
PROCESS: Complete homogenization and release of DNA for
polymerize chain-reaction
PREVIOUS OR CURRENT METHOD: Grinding with glass rod or
applicator sticks
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: Worked well in grinding the fruit flies
and preservation of DNA, however, scientists were concerned over
the mixing of DNA molecules from different strains as material
stuck to the generators during processing.
RECOMMENDATIONS: Generators can be completely immersed and
sterilized between applications with minimal disassembly.

Ecoli cells
PRODUCT APPLICATION REPORT
MATERIAL: E. Coli cells
VOLUME OF MATERIAL: 25 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Breakage of cell (100%)
PREVIOUS OR CURRENT METHOD: Ultrasonic homogenizer
EQUIPMENT USED:
PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: Did not get 100% breakage in 15 seconds.
Sometimes longer running times are needed to
obtain complete homogenization. Fifteen seconds is not quite long
enough for the type of disruption needed for this application.

Escherichia coil
PRODUCT APPLICATION REPORT
MATERIAL: Escherichia coli
VOLUME OF MATERIAL: Not reported
MEDIUM: Chemical solution
VOLUME OF MEDIUM: Not reported
PROCESS: Isolation of fimbriae
PREVIOUS OR CURRENT METHOD: None
EQUIPMENT USED:
PRO200 Homogenizer with 20mm generator
APPLICATION AND RESULTS: The 20mm generator produced the required
homogenization result.

Fall Armyworm
PRODUCT APPLICATION REPORT

MATERIAL: Fall Armyworm
VOLUME OF MATERIAL:
MEDIUM: 4 ml of phosphate buffered saline
VOLUME OF MEDIUM: 15 ml tube
PROCESS: Homogenized
PREVIOUS OR CURRENT METHOD:
EQUIPMENT USED: PRO Homogenizer 
APPLICATION AND RESULTS:

DOI:		 Individual speciments were homogenized in 4 ml of phosphate buffered saline in a 15ml test tube using a tissue homogenizer. 

http://dx.doi.org/10.1653/024.096.0305

Fig Newton cookies
PRODUCT APPLICATION REPORT
MATERIAL: Fig Newton cookies
VOLUME OF MATERIAL: 20 grams
MEDIUM: None
VOLUME OF MEDIUM: Not reported
PROCESS: Grinding to measure moisture
PREVIOUS OR CURRENT METHOD: Waring blender
EQUIPMENT USED: PRO250 Homogenizer w/stand or PRO300 Homogenizer with PRO Safety Sealed Chamber Assembly
APPLICATION AND RESULTS: Able to grind the Fig Newton, but lack of
medium caused the paste within the cookie to become heated and
reduces the moisture content, which had comparable results to the
blender. A medium is recommended.

Fish oil
PRODUCT APPLICATION REPORT
MATERIAL: Fish oil
VOLUME OF MATERIAL: Not reported
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenize for future application
PREVIOUS OR CURRENT METHOD: Mechanical and ultrasonic homogenizer
EQUIPMENT USED: PRO250 Homogenizer with speed control box
APPLICATION AND RESULTS:



DOI:		 The hot emulsion resulted by mixing the lipid 
and aqueous phases at 85°C was exposed 
to an external mechanical energy by high shear homogenization.

http://www.scientificbulletin.upb.ro/rev_docs_arhiva/rezc5e_246325.pdf


Fish tissue
PRODUCT APPLICATION REPORT
MATERIAL: Fish tissue
VOLUME OF MATERIAL: 10-400 ml
MEDIUM: Water
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenize for future application
PREVIOUS OR CURRENT METHOD: Hand and ultrasonic homogenizer
EQUIPMENT USED: PRO250 Homogenizer w/stand or PRO300 Homogenizer with PRO Safety Sealed Chamber Assembly
APPLICATION AND RESULTS: Worked well and sealed system proved to
be essential success of application.

Food additives
PRODUCT APPLICATION REPORT
MATERIAL: Food additives
VOLUME OF MATERIAL: various
MEDIUM: : 1) Mayonnaise 2) Salad dressing 1 gallon (PRO200 Homogenizer  Process)
VOLUME OF MEDIUM: 1 gallon
PROCESS: Mix food product while adding various additives in liquid
and powder form to obtain vertical flow.
PREVIOUS OR CURRENT METHOD: Waring blender
EQUIPMENT USED: PRO250 Homogenizer w/stand & 20mm probe or PRO300 Homogenizer with PRO Safety Sealed Chamber Assembly
APPLICATION AND RESULTS: The PRO200 Homogenizer  and the 20mm generator were
unable to mix the mayonnaise and the secret additive, however it
worked superbly on the salad dressing. The PRO300 D Homogenizer and the blade
system were used on the mayonnaise and began to mix but there was
no vertical flow of material.

RECOMMENDATIONS: Recommend that a larger diameter generator and
PRO250 Homogenizer or PRO300 D Homogenizer be used to cause vertical flow of the material.

Food staples
PRODUCT APPLICATION REPORT
MATERIAL: Food samples
VOLUME OF MATERIAL: 1 ml
MEDIUM: Organic aqueous
VOLUME OF MEDIUM: 5 ml
PROCESS: Complete dispersion
PREVIOUS OR CURRENT METHOD: None
EQUIPMENT USED: PRO200 Homogenizer with 7mm or 10mm generator
APPLICATION AND RESULTS: Good homogenization results

Freshwater snail
PRODUCT APPLICATION REPORT
MATERIAL: Freshwater snail
VOLUME OF MATERIAL: Not reported
MEDIUM: distilled water
VOLUME OF MEDIUM: 0.5 ml
PROCESS: Homogenized
PREVIOUS OR CURRENT METHOD:
EQUIPMENT USED: PRO200 Homogenizer  
APPLICATION AND RESULTS:

DOI:		 Each body was weighed, transferred to a flat bottom tube and homogenized for 15 s. 

http://dx/doi.org/10.1080/00288330.2013.846921

Frog embryos
PRODUCT APPLICATION REPORT
MATERIAL: Frog embryos
VOLUME OF MATERIAL: Not reported
MEDIUM: varied
VOLUME OF MEDIUM: 1.5 ml
PROCESS: Cell disruption
PREVIOUS OR CURRENT METHOD: Mortar and pestle
EQUIPMENT USED: PRO200 Homogenizer with 7mm or 10mm generator
APPLICATION AND RESULTS: Worked well.

Frozen rat heart
PRODUCT APPLICATION REPORT
MATERIAL: Frozen rat heart
VOLUME OF MATERIAL: 130-150 mg
MEDIUM: HEPES, EDTA, DTT, MgCl2
VOLUME OF MEDIUM: 25-30mM
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer
APPLICATION AND RESULTS: Achieved desired results
Link DOI: http://dx.doi.org/10.1002/jcp.24079

Fungi
PRODUCT APPLICATION REPORT
MATERIAL: 50 ml
VOLUME OF MATERIAL: Not reported
MEDIUM: Not reported
VOLUME OF MEDIUM: 50 ml
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: Worked well.

Gypsy Moth eggs
PRODUCT APPLICATION REPORT
MATERIAL: Gypsy Moth eggs
VOLUME OF MATERIAL: 05 ml
MEDIUM: Aqueous
VOLUME OF MEDIUM: 1 ml
PROCESS: Rupture of eggs
PREVIOUS OR CURRENT METHOD: Manual
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: Successfully ruptured eggs for further
analysis.

Hair shampoo
PRODUCT APPLICATION REPORT
MATERIAL: Hair shampoo
VOLUME OF MATERIAL: 15000-20000g
MEDIUM: None
VOLUME OF MEDIUM: None
PROCESS: Mix shampoo to desired consistency
PREVIOUS OR CURRENT METHOD: Large mixer with 24" rotor and
4" blade
EQUIPMENT USED: PRO250 Homogenizer w/stand & 20mm probe or PRO300 Homogenizer with PRO Safety Sealed Chamber Assembly
APPLICATION AND RESULTS: The blade moved a great deal of the
shampoo because the high rpms.

Hamster brain
PRODUCT APPLICATION REPORT
MATERIAL: Hamster brain
VOLUME OF MATERIAL: 10 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization for RNA and protein purification
analysis
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: Successful completion of application.

Hamster tissue
PRODUCT APPLICATION REPORT
MATERIAL: Hamster tissue
VOLUME OF MATERIAL: 9 grams
MEDIUM: Aqueous
VOLUME OF MEDIUM: 1-5 ml
PROCESS: Release bacteria from tissue
PREVIOUS OR CURRENT METHOD: None
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: Required results achieved easily.

Heart cells
PRODUCT APPLICATION REPORT
MATERIAL: Heart cells
VOLUME OF MATERIAL: Not reported
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Isolation of metabolites
PREVIOUS OR CURRENT METHOD: None
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: Worked well. Intended results achieved
within seconds.

Hippocampus
PRODUCT APPLICATION REPORT
MATERIAL: Rat hippocampus
VOLUME OF MATERIAL: Not reported
MEDIUM: Cocktail lysis buffer containing protease 
inhibitors and phosphatase inhibitors
VOLUME OF MEDIUM: Not reported
PROCESS: Snap-frozen in liquid nitrogen and homogenized
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer 
APPLICATION AND RESULTS:
DOI:		 Achieved desired homogenization results
http://dx.doi.org/10.1016/j.neuropharm.2014.10.011


Hippocampus and prefrontal cortex 
PRODUCT APPLICATION REPORT
MATERIAL: Hippocampus and prefrontal cortex 
VOLUME OF MATERIAL: 1 ml
MEDIUM:
VOLUME OF MEDIUM: Not reported
PROCESS:
PREVIOUS OR CURRENT METHOD: None
EQUIPMENT USED: PRO Homogenizer
APPLICATION AND RESULTS:

DOI:		 Hippocampus and prefrontol cortex and 
1.0ml for nucleus accumbens, using a handheld homogenizer on slow setting.  

http://dx.doi.org/10.1007/s00216-013-7514-9

HIV Infected H9 cells
PRODUCT APPLICATION REPORT
MATERIAL: HIV Infected H9 cells
VOLUME OF MATERIAL: 20-200 g
MEDIUM: Not reported
VOLUME OF MEDIUM: 50-400 ml
PROCESS: Disrupt cell membrane
PREVIOUS OR CURRENT METHOD: Dounce Homogenizer
EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer with blade and sealed chamber assembly
APPLICATION AND RESULTS: Worked well and sealed system is a
must. However, sharpened blades may pose a huge health risk if
poked into skin.

RECOMMENDATIONS: As an added safety feature for working with a
such material, we would suggest the use of a generator system
(smooth bottomed) that has no exposed sharp edges that can break
the skin of its user.

Human liver
PRODUCT APPLICATION REPORT
MATERIAL: Human liver
VOLUME OF MATERIAL: 100-200 ml
MEDIUM: Water
VOLUME OF MEDIUM: Not reported
PROCESS: Complete homogenization
PREVIOUS OR CURRENT METHOD: None
EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer with blade and blade and probe sealed chamber assembly and 20mm probe 
APPLICATION AND RESULTS: Worked best with the blade and sealed
chamber assembly. Chunks were too big for the 20mm generator.

Human tissue
PRODUCT APPLICATION REPORT
MATERIAL: Human tissue
VOLUME OF MATERIAL: 5 g
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Brinkmannn homogenizer
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: Homogenized tissue well and was quieter
than previous methods.

Human tissue
PRODUCT APPLICATION REPORT
MATERIAL: Human tissue
VOLUME OF MATERIAL: .05 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenize RNA
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: Worked well

Human tissue
PRODUCT APPLICATION REPORT
MATERIAL: Human tissue
VOLUME OF MATERIAL: 8 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: RNA extraction
PREVIOUS OR CURRENT METHOD: Brinkmannn Polytron
EQUIPMENT USED: PRO200 Homogenizer with 7mm generator
APPLICATION AND RESULTS: Liked low noise level of units. Achieved
desired results.

Human tissue
PRODUCT APPLICATION REPORT
MATERIAL: Human tissue
VOLUME OF MATERIAL: 20 ml
MEDIUM: Phosphate buffer
VOLUME OF MEDIUM: 50 ml
PROCESS: Complete homogenization
PREVIOUS OR CURRENT METHOD: shift blender, mortar and pestle
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: Worked very well and desired results were
achieved.

Intestinal Samples (Swine)
PRODUCT APPLICATION REPORT


MATERIAL: Intestinal samples (Swine)
VOLUME OF MATERIAL: 2mm pieces
MEDIUM:
VOLUME OF MEDIUM:
PROCESS: Lysed
PREVIOUS OR CURRENT METHOD:
EQUIPMENT USED: PRO200 Homogenizer
APPLICATION AND RESULTS:


DOI:		 To obtain representative measurements from the
2-cm jejunal samples, three cross-sections of 
approximately 2 mm were pooled and lysed using
PRO 200 Post-Mounted Laboratory

http://dx.doi.org/10.1371/journal.pone.0079343


Keratinocytes 
PRODUCT APPLICATION REPORT
MATERIAL: Keratinocytes 
VOLUME OF MATERIAL: Not reported
MEDIUM:
VOLUME OF MEDIUM: Not reported
PROCESS:
PREVIOUS OR CURRENT METHOD: None
EQUIPMENT USED: PRO200 Homogenizer
APPLICATION AND RESULTS:

DOI:		 Successful homogenization  

http://dx.doi.org/10.1038/ncomms6230

Keratinocytes 
PRODUCT APPLICATION REPORT
MATERIAL: Psoriatic keratinocytes 
VOLUME OF MATERIAL: Not reported
MEDIUM: TRIzol reagent
VOLUME OF MEDIUM: Not reported
PROCESS:
PREVIOUS OR CURRENT METHOD: None
EQUIPMENT USED: PRO Homogenizer
APPLICATION AND RESULTS:

DOI:		 Successful homogenization  

http://dx.doi.org/10.1046/j.1523-1747.2002.19509.x

Keratinocytes 
PRODUCT APPLICATION REPORT
MATERIAL: Dog keratinocytes 
VOLUME OF MATERIAL: Not reported
MEDIUM: RNALater
VOLUME OF MEDIUM: Not reported
PROCESS:
PREVIOUS OR CURRENT METHOD: None
EQUIPMENT USED: PRO200 Homogenizer w/Multi-Gen 7
APPLICATION AND RESULTS:

DOI:		 Successful homogenization  

http://dx.doi.org/10.1111/vde.12034

Kidneys
PRODUCT APPLICATION REPORT


MATERIAL: Kidneys
VOLUME OF MATERIAL:
MEDIUM: PBS
VOLUME OF MEDIUM: 5 ml
PROCESS: Complete homogenization
PREVIOUS OR CURRENT METHOD:
EQUIPMENT USED: PRO Homogenizer
APPLICATION AND RESULTS:


DOI:		 Kidneys excised for fungal burden assessments 
were weighed, placed in 5 ml PBS, and homogenized
for 30 s with a tissue homogenizer. 

http://dx.doi.org/10.1128/mBio.00723-13

Kidneys
PRODUCT APPLICATION REPORT


MATERIAL: Kidneys
VOLUME OF MATERIAL:
MEDIUM:
VOLUME OF MEDIUM:
PROCESS: For Western blotting, grinded, quantified and then homogenized. 
PREVIOUS OR CURRENT METHOD:
EQUIPMENT USED: PRO Homogenizer
APPLICATION AND RESULTS:


DOI:		 To investigate HSP expression, some portion of the isolated right and left kidneys were cut into
pieces and stored at −72°C. For Western blotting, the stored kidney pieces were grinded, quantified, and then homogenized. 

http://dx/doi.org/10.1016/j.transproceed.2013.08.028


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