PRO Scientific Homogenizer Applications from L–R
Explore laboratory homogenizer application reports for materials beginning with L through R. These application notes include sample material, volume, medium, processing method, equipment used, and reported results from PRO Scientific homogenizer applications.
L Applications
Larval samples
| Volume Of Material | Not reported |
|---|---|
| Medium | cold Tri- HCl buffer |
| Volume Of Medium | 4:1 v/w |
| Process | Homogenized |
| Previous Or Current Method | Not reported |
| Equipment Used | PRO200 Homogenizer |
| Application and Results | Larval samples were homogenized using a tissue homogenizer. |
Latex particles
| Volume Of Material | Not reported |
|---|---|
| Medium | Dispersed and continuous phase |
| Volume Of Medium | Ratio of 1:3 |
| Process | Successive homogenization at 14,500 rpm for 40 seconds and 21,000 rpm for 20 seconds |
| Previous Or Current Method | Not reported |
| Equipment Used | PRO200 Homogenizer |
| Application and Results | Successful homogenization https://doi.org/10.1080/01932691.2014.966310 |
Leaves
| Volume Of Material | 25 ml |
|---|---|
| Medium | Organic buffer |
| Volume Of Medium | 75 ml |
| Process | Grinding of leaves for protein analysis |
| Previous Or Current Method | Brinkmannn Polytron |
| Equipment Used | PRO200 Homogenizer with 10mm generator |
| Application and Results | Good results. |
Lipid and aqueous phases
| Volume Of Material | Not reported |
|---|---|
| Medium | Lipid and aqueous phases |
| Volume Of Medium | Not reported |
| Process | Homogenized for 5 minutes |
| Previous Or Current Method | Not reported |
| Equipment Used | PRO250 Homogenizer |
| Application and Results | Blended by a high-shear homogenizer |
Lipid and aqueous phases
| Volume Of Material | Not reported |
|---|---|
| Medium | Lipid and aqueous phases |
| Volume Of Medium | Not reported |
| Process | Homogenized for 5 minutes |
| Previous Or Current Method | Not reported |
| Equipment Used | PRO250 Homogenizer |
| Application and Results | Aqueuous phase was added to lipid phase and mixed under homogenization at 12,000 rpm with PRO250. |
Liver cells from rat
| Volume Of Material | 10 ml |
|---|---|
| Medium | Krebs-Ringer buffer |
| Volume Of Medium | 20 ml |
| Process | Rupturing of hepatocytes and leaving of sub-cellular components intact |
| Previous Or Current Method | Dounce homogenizer |
| Equipment Used | PRO200 Homogenizer with 10mm generator |
| Application and Results | The PRO200 Homogenizer ruptured the cells in a very short time (2 minutes) leaving the sub-cellular components intact. There was minimal foaming and the compactness and noise level of the instrument were added benefits |
Liver tissue (mouse)
| Volume Of Material | .5-5 ml |
|---|---|
| Medium | PBS |
| Volume Of Medium | Not reported |
| Process | Not reported |
| Previous Or Current Method | Not reported |
| Equipment Used | PRO Homogenizer |
| Application and Results | Liver tissue was homogenized on ice for 30 s. |
Lymphocyte, brain, and thymus
| Volume Of Material | .5-5 ml |
|---|---|
| Medium | GITC buffer |
| Volume Of Medium | Not reported |
| Process | Isolation of RNA and DNA |
| Previous Or Current Method | Mortar and pestle, blender |
| Equipment Used | PRO200 Homogenizer with 5mm or 7mm generator |
| Application and Results | Application successful with either generator between the volumes of material. |
M Applications
Mice lungs
| Medium | 50 ml |
|---|---|
| Volume Of Medium | 100 ml |
| Process | Homogenization |
| Previous Or Current Method | Virtis |
| Equipment Used | PRO200 Homogenizer with 10mm generator |
| Application and Results | Heating was a concern, but none resulted and the experiment was completed successfully. |
Mango seed kernel
| Volume Of Material | Not reported |
|---|---|
| Medium | Not reported |
| Volume Of Medium | Not reported |
| Process | Effects of encapsulation materials, including polysaccharides and proteins |
| Previous Or Current Method | Not Reported |
| Equipment Used | PRO200 Homogenizer |
| Application and Results | 10,000rpm for 2 minutes |
| DOI | https://doi.org/10.1016/j.foodchem.2012.03.054 |
Mosquitoes
| Volume Of Material | 50 mosquitoes |
|---|---|
| Medium | Not reported |
| Volume Of Medium | Not reported |
| Process | Virus analysis in mosquitoes |
| Previous Or Current Method | Mortar and pestle |
| Equipment Used | PRO200 Homogenizer with 5mm generator |
| Application and Results | PRO200 saves considerable amount of time and works well in this application. |
Mouse ears
| Volume Of Material | 1-5 ml |
|---|---|
| Medium | Buffer solution |
| Volume Of Medium | Not reported |
| Process | Cell disruption |
| Previous Or Current Method | Brinkmannn Polytron |
| Equipment Used | PRO200 Homogenizer with 5 or 7mm generator |
| Application and Results | Cell disruption and adequate sample size achieved with both generator sizes (generator diameter depended on material volume), which had considerably less noise than Polytron. |
Mouse liver, spleen, lung, and skin
| Volume Of Material | 1 cm2 |
|---|---|
| Medium | sterile PBS |
| Volume Of Medium | 2 ml |
| Process | Homogenization |
| Previous Or Current Method | Not reported |
| Equipment Used | PRO200 Homogenizer |
| Application and Results | Achieved desired homogenization results for overnight incubation/ colonization |
Mouse lung, spleen, and liver
| Volume Of Material | Not reported |
|---|---|
| Medium | sterile saline |
| Volume Of Medium | Not reported |
| Process | Homogenized at 4°C |
| Previous Or Current Method | Not reported |
| Equipment Used | PRO200 Homogenizer |
| Application and Results | Achieved desired homogenization results https://doi.org/10.1186/s12879-014-0559-3 |
Mouse muscle
| Volume Of Material | 2 ml |
|---|---|
| Medium | Body fluids |
| Volume Of Medium | 4 ml |
| Process | Not reported |
| Previous Or Current Method | Mortar and Pestle |
| Equipment Used | PRO200 Homogenizer with 7mm generator |
| Application and Results | Results were acceptable |
Mouse organs
| Volume Of Material | 10 ml |
|---|---|
| Medium | Not reported |
| Volume Of Medium | Not reported |
| Process | Homogenization |
| Previous Or Current Method | Stirring motor with grinder |
| Equipment Used | PRO200 Homogenizer with 10mm generator |
| Application and Results | The PRO200 Homogenizer successfully completed the application. |
Mouse skin
| Volume Of Material | 200 mg |
|---|---|
| Medium | Buffered salt solution |
| Volume Of Medium | 0.5 m1 |
| Process | Thoroughly homogenize mouse skin to do Beta Galactosidase test. |
| Previous Or Current Method | Freeze in liquid nitrogen and grind by hand. |
| Equipment Used | PRO200 Homogenizer with 7mm generator |
| Application and Results | Results were obtained much faster than by hand and the quantitation numbers compared to the old method of processing by hand. |
Muscle brain connective tissue
| Volume Of Material | 50ml |
|---|---|
| Medium | Water |
| Volume Of Medium | 100 ml |
| Process | Pulverize or powder frozen muscle tissue |
| Previous Or Current Method | Tekmar Tissu-mizer |
| Equipment Used | PRO250 Homogenizer (w/ stand) or PRO300D a 20mm generator |
| Application and Results | Generator was able to provide successful results. |
Muscle tissue
| Volume Of Material | <.5 ml="" td=""> |
|---|---|
| Medium | Aqueous buffer |
| Volume Of Medium | 2 ml |
| Process | Extraction |
| Previous Or Current Method | Mortar and pestle after freezing sample with liquid nitrogen |
| Equipment Used | PRO200 Homogenizer with 5mm or 7mm generator |
| Application and Results | Both generators work within an Eppendorf tube. Both generators produce the same good results, because of the small volume of material. Emulsion will exists in larger samples. |
Mustard and pepper buds
| Volume Of Material | 20 total buds |
|---|---|
| Medium | Buffer |
| Volume Of Medium | 30 ml |
| Process | Release of microspores and removal of supernatant |
| Previous Or Current Method | Mortar and pestle |
| Equipment Used | PRO200 Homogenizer with 10mm generator |
| Application and Results | Dispersion was more than adequate. |
N Applications
Nerve segments
| Volume Of Material | .1 ml (5mm gen.) and 75 ml (10mm gen.) |
|---|---|
| Medium | SDS buffer |
| Volume Of Medium | Not reported |
| Process | Homogenization |
| Previous Or Current Method | Manual grinding and glass-glass microhomogenizer |
| Equipment Used | PRO200 Homogenizer with 5 & 10mm generator |
| Application and Results | Application successful. |
Nerve tissue
| Volume Of Material | .1 ml |
|---|---|
| Medium | Not reported |
| Volume Of Medium | Not reported |
| Process | Homogenization |
| Previous Or Current Method | Wheaton mortar and pestle |
| Equipment Used | PRO200 Homogenizer with 5mm generator |
| Application and Results | Tissue was too stringy and offered no resistance to the blade. RECOMMENDATIONS: In situations where the material is too flexible or stringy, liquid nitrogen can be used to freeze the sample. The blade will then be able to cut through the frozen sample. The liquid nitrogen allows for the sample to be ground very finely. A 7mm saw tooth bottom generator would also be recommended when liquid nitrogen is not available. |
Nuts
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