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What I'm Homogenizing L-R

PRO Homogenizer Applications from L - R 


Larval samples
PRODUCT APPLICATION REPORT
MATERIAL: Larval samples
VOLUME OF MATERIAL:
MEDIUM: cold Tri- HCl buffer
VOLUME OF MEDIUM: 4:1 v/w 
PROCESS: Homogenized
PREVIOUS OR CURRENT METHOD:
EQUIPMENT USED: PRO200 Homogenizer   
APPLICATION AND RESULTS:

DOI:		 Larval samples were homogenized using a tissue homogenizer.

http://dx/doi.org/10.1007/s10695-013-9884-5
Latex particles
PRODUCT APPLICATION REPORT

MATERIAL: Latex particles
VOLUME OF MATERIAL: Not reported
MEDIUM: Dispersed and continuous phase 
VOLUME OF MEDIUM: Ratio of 1:3
PROCESS: Successive homogenization at 14,500 rpm for 
40 seconds and 21,000 rpm for 20 seconds
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer  
APPLICATION AND RESULTS:


DOI:


 Successful homogenization

http://dx.doi.org/10.1080/01932691.2014.966310

Leaves
PRODUCT APPLICATION REPORT
MATERIAL: Leaves
VOLUME OF MATERIAL: 25 ml
MEDIUM: Organic buffer
VOLUME OF MEDIUM: 75 ml
PROCESS: Grinding of leaves for protein analysis
PREVIOUS OR CURRENT METHOD: Brinkmannn Polytron
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: Good results.

Lipid and aqueous phases
PRODUCT APPLICATION REPORT
MATERIAL: Lipid and aqueous phases
VOLUME OF MATERIAL: Not reported
MEDIUM: Lipid and aqueous phases
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenized for 5 minutes
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO250 Homogenizer
APPLICATION AND RESULTS: Blended by a high-shear homogenizer

Lipid and aqueous phases
PRODUCT APPLICATION REPORT
MATERIAL: Lipid and aqueous phases
VOLUME OF MATERIAL: Not reported
MEDIUM: Lipid and aqueous phases
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenized for 5 minutes
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO250 Homogenizer
APPLICATION AND RESULTS:

DOI:		 Aqueuous phase was added to lipid phase and mixed under homogenization at 12,000 rpm with PRO250.  

http://dx.doi.org/10.1208/s12248-013-9550-y


Liver cells from rat
PRODUCT APPLICATION REPORT
MATERIAL: Liver cells from rat
VOLUME OF MATERIAL: 10 ml
MEDIUM: Krebs-Ringer buffer
VOLUME OF MEDIUM: 20 ml
PROCESS: Rupturing of hepatocytes and leaving of sub-cellular
components intact
PREVIOUS OR CURRENT METHOD: Dounce homogenizer
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: The PRO200 Homogenizer  ruptured the cells in a very
short time (2 minutes) leaving the sub-cellular components
intact. There was minimal foaming and the compactness and noise
level of the instrument were added benefits

Liver tissue (mouse)
PRODUCT APPLICATION REPORT
MATERIAL: Liver tissue (mouse)
VOLUME OF MATERIAL: .5-5 ml
MEDIUM: PBS
VOLUME OF MEDIUM: Not reported
PROCESS:
PREVIOUS OR CURRENT METHOD:
EQUIPMENT USED: PRO Homogenizer
APPLICATION AND RESULTS:

DOI:		 Liver tissue was homogenized on ice for 30 s. 

http://dx/doi.org/10.1021/jf403173k

Lymphocyte brain and thymus
PRODUCT APPLICATION REPORT
MATERIAL: Lymphocyte, brain, and thymus
VOLUME OF MATERIAL: .5-5 ml
MEDIUM: GITC buffer
VOLUME OF MEDIUM: Not reported
PROCESS: Isolation of RNA and DNA
PREVIOUS OR CURRENT METHOD: Mortar and pestle, blender
EQUIPMENT USED: PRO200 Homogenizer with 5mm or 7mm generator
APPLICATION AND RESULTS: Application successful
with either generator between the volumes of
material.

Mice lungs
PRODUCT APPLICATION REPORT
MATERIAL: Mice lungs
MEDIUM: 50 ml
VOLUME OF MEDIUM: 100 ml
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Virtis
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: Heating was a concern, but none resulted
and the experiment was completed successfully.

Mango seed kernel

PRODUCT APPLICATION REPORT
MATERIAL: Mango seed kernel
VOLUME OF MATERIAL: Not reported
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Effects of encapsulation materials, including polysaccharides and proteins
PREVIOUS OR CURRENT METHOD: Not Reported
EQUIPMENT USED: PRO200 Homogenizer 
APPLICATION AND RESULTS: 10,000rpm for 2 minutes 
Link DOI: http://dx.doi.org/10.1016/j.foodchem.2012.03.054

Mosquitoes

PRODUCT APPLICATION REPORT
MATERIAL: Mosquitoes
VOLUME OF MATERIAL: 50 mosquitoes
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Virus analysis in mosquitoes
PREVIOUS OR CURRENT METHOD: Mortar and pestle
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: PRO200 saves considerable amount of time
and works well in this application.

Mouse ears
PRODUCT APPLICATION REPORT
MATERIAL: Mouse ears
VOLUME OF MATERIAL: 1-5 ml
MEDIUM: Buffer solution
VOLUME OF MEDIUM: Not reported
PROCESS: Cell disruption
PREVIOUS OR CURRENT METHOD: Brinkmannn Polytron
EQUIPMENT USED: PRO200 Homogenizer with 5 or 7mm generator
APPLICATION AND RESULTS: Cell disruption and adequate sample size
achieved with both generator sizes (generator diameter depended on
material volume), which had considerably less noise than Polytron.

Mouse liver, spleen, lung, and skin
PRODUCT APPLICATION REPORT
MATERIAL: Mouse liver, spleen, lung, and skin
VOLUME OF MATERIAL: 1 cm2
MEDIUM: sterile PBS
VOLUME OF MEDIUM: 2 ml
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer 
APPLICATION AND RESULTS: Achieved desired homogenization results for overnight incubation/ colonization 

Mouse lung, spleen, and liver
PRODUCT APPLICATION REPORT
MATERIAL: Mouse lung, spleen, and liver
VOLUME OF MATERIAL:
MEDIUM: sterile saline
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenized at 4°C 
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer 
APPLICATION AND RESULTS:
DOI:		 Achieved desired homogenization results 
http://dx.doi.org/10.1186/s12879-014-0559-3

Mouse muscle
PRODUCT APPLICATION REPORT
MATERIAL: Mouse muscle
VOLUME OF MATERIAL: 2 ml
MEDIUM: Body fluids
VOLUME OF MEDIUM: 4 ml
PROCESS: Not reported
PREVIOUS OR CURRENT METHOD: Mortar and Pestle
EQUIPMENT USED: PRO200 Homogenizer with 7mm generator
APPLICATION AND RESULTS: Results were acceptable

Mouse organs
PRODUCT APPLICATION REPORT
MATERIAL: Mouse organs
VOLUME OF MATERIAL: 10 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Stirring motor with grinder
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: The PRO200 Homogenizer  successfully completed the
application.

Mouse skin
PRODUCT APPLICATION REPORT
MATERIAL: Mouse skin
VOLUME OF MATERIAL: 200 mg
MEDIUM: Buffered salt solution
VOLUME OF MEDIUM: 0.5 m1
PROCESS: Thoroughly homogenize mouse skin to do Beta Galactosidase test.
PREVIOUS OR CURRENT METHOD: Freeze in liquid nitrogen and grind by hand.
EQUIPMENT USED: PRO200 Homogenizer with 7mm generator
APPLICATION AND RESULTS: Results were obtained much faster than by
hand and the quantitation numbers compared to the old method of
processing by hand.

Muscle brain connective tissue
PRODUCT APPLICATION REPORT
MATERIAL: Muscle brain connective tissue
VOLUME OF MATERIAL: 50ml
MEDIUM: Water
VOLUME OF MEDIUM: 100 ml
PROCESS: Pulverize or powder frozen muscle tissue
PREVIOUS OR CURRENT METHOD: Tekmar Tissu-mizer
EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300D a  20mm generator
APPLICATION AND RESULTS: Generator was able to provide successful
results.

Muscle tissue
PRODUCT APPLICATION REPORT
MATERIAL: Muscle tissue
VOLUME OF MATERIAL: <.5 ml
MEDIUM: Aqueous buffer
VOLUME OF MEDIUM: 2 ml
PROCESS: Extraction
PREVIOUS OR CURRENT METHOD: Mortar and pestle after freezing
sample with liquid nitrogen
EQUIPMENT USED: PRO200 Homogenizer with 5mm or 7mm generator
APPLICATION AND RESULTS: Both generators work within an
Eppendorf tube. Both generators produce the same good
results, because of the small volume of material. Emulsion will
exists in larger samples.

Mustard and pepper buds
PRODUCT APPLICATION REPORT
MATERIAL: Mustard and pepper buds
VOLUME OF MATERIAL: 20 total buds
MEDIUM: Buffer
VOLUME OF MEDIUM: 30 ml
PROCESS: Release of microspores and removal of supernatant
PREVIOUS OR CURRENT METHOD: Mortar and pestle
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: Dispersion was more than adequate.

Nerve segments
PRODUCT APPLICATION REPORT
MATERIAL: Nerve segments
VOLUME OF MATERIAL: .1 ml (5mm gen.) and 75 ml (10mm gen.)
MEDIUM: SDS buffer
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Manual grinding and glass-glass
microhomogenizer
EQUIPMENT USED: PRO200 Homogenizer with 5 & 10mm generator
APPLICATION AND RESULTS: Application successful.

Nerve tissue
PRODUCT APPLICATION REPORT
MATERIAL: Nerve tissue
VOLUME OF MATERIAL: .1 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Wheaton mortar and pestle
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: Tissue was too stringy and offered no
resistance to the blade.
RECOMMENDATIONS: In situations where the material is too flexible
or stringy, liquid nitrogen can be used to freeze the sample. The
blade will then be able to cut through the frozen sample. The
liquid nitrogen allows for the sample to be ground very finely. A 7mm saw tooth bottom generator would also be recommended when
liquid nitrogen is not available.

Nuts
PRODUCT APPLICATION REPORT
MATERIAL: Nuts
VOLUME OF MATERIAL: 1 g
MEDIUM: Water
VOLUME OF MEDIUM: 20 ml
PROCESS: Homogenization to recover enzymes
PREVIOUS OR CURRENT METHOD: Mortar and pestle and then vortex
stirrer
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: Nuts were ground successfully.

Olfactory bulb
PRODUCT APPLICATION REPORT
MATERIAL: Olfactory bulb
VOLUME OF MATERIAL:
MEDIUM: Protease inhibitors were added to a homogenization buffer
VOLUME OF MEDIUM:
PROCESS:
PREVIOUS OR CURRENT METHOD:
EQUIPMENT USED: PRO Homogenizer  
APPLICATION AND RESULTS:

DOI:		 Samples were homogenized by 50 strokes on ice.

http://dx.doi.org/10.1016/j.neuroscience.2013.12.033

Ovarian tissue
PRODUCT APPLICATION REPORT
MATERIAL: Ovarian tissue
VOLUME OF MATERIAL: 10 ml
MEDIUM: Phosphate buffer
VOLUME OF MEDIUM: Not reported
PROCESS: RNA and mitochondria isolation
PREVIOUS OR CURRENT METHOD: bounce homogenizer, Brinkmann
Polytron
EQUIPMENT USED: PRO200 Homogenizer with 7mm generator
APPLICATION AND RESULTS: Very good for homogenizing small amounts
of tissue. Broke up 75% of cells in the defined protocol.

Pancreas virus tissue
PRODUCT APPLICATION REPORT

MATERIAL: Pancreas virus tissue
VOLUME OF MATERIAL: 1 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Extraction
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: The PRO200 worked excellently

Parasites
PRODUCT APPLICATION REPORT

MATERIAL: Proteins extracted from parasites
VOLUME OF MATERIAL:
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Extraction
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer
APPLICATION AND RESULTS: Proteins were extracted from the parasites by immersing the parasites in an ice bath, followed by homogenization with a hand-held PRO200

Peanuts and Mumg beans
PRODUCT APPLICATION REPORT
MATERIAL: Peanuts and Mung beans
VOLUME OF MATERIAL: 50 ml
MEDIUM: Aqueous
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization of hypocotyls and seeds to isolate protein
PREVIOUS OR CURRENT METHOD: Brinkmannn Polytron
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: Dispersal achieved successfully for the
protein analysis.

Peanut butter defatted and dried
PRODUCT APPLICATION REPORT
MATERIAL: Peanut butter, defatted and dried
VOLUME OF MATERIAL: 40 ml of unpacked material
MEDIUM: None
VOLUME OF MEDIUM: None
PROCESS: Process this material to a level of consistent
particle size-ideally particle size of this material should pass thru a 40 mesh.
PREVIOUS OR CURRENT METHOD: Unknown
EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300D with the following:

T1: 20mm and 30mm generator run in a 150m1 glass beaker.

T2: 30mm generator run in a 150m1 glass beaker.

T3: 30mm generator run in a 150m1 glass beaker.
APPLICATION AND RESULTS: Various tests were run as follows:

Test 1: Ran at 28,000 rpm for 1 minute – up and down. Stopped. Ran at 28,000 rpm for 1 minute – up and down.

Test 2: Ran at 10,000 rpm for 1 minute. Stopped. Ran at 28,000 rpm for 1 minute.

Test 3: Ran at 28,000 rpm for 1-½ minutes.
RECOMMENDATIONS:

Test 1: The 30mm generator seemed to work as well as the 20mm generator, so we would recommend using only one generator.

Test 2: This test proved to us that running just the 30mm generator for 2 minutes, was as good as running both a 20mm as well as a 30mm generator for 2 minutes.

Test 3: This test was done to determine the level of homogenization that was done in 1 1/2 minutes compared to the samples in tests 1 and 2, which were run for 2 minutes. Same
results.

Peanut butter defatted and dried
PRODUCT APPLICATION REPORT
MATERIAL: Peanut butter, defatted and dried
VOLUME OF MATERIAL: 120 ml of unpacked material.
MEDIUM: None
VOLUME OF MEDIUM: None
PROCESS: Process this material to a level of consistent particle size - ideally this material should pass thru a 40 mesh.
PREVIOUS OR CURRENT METHOD: Unknown
EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300D with a 2" diameter blade attached to a 473m1 sealed glass container.
APPLICATION AND RESULTS: Test 1: 120m1, 28,000 rpm for 30 sec.
Stop. Remove and shake the container, 28,000 rpm for 30 sec. Stop.

Test 2: 120m1 at 28,000 rpm for 20 sec. Stop. Remove and shake the container. Remove all but 40m1 of material. Run at 28,000 rpm for 20 sec.

Test 3: Balance of 120m1 from test 2 at 28,000 rpm for 20 seconds. Then in a 50 m1 conical tube at 28,000 rpm for 45 seconds.

RECOMMENDATIONS:
1: This method of using a sealed container with a blade was better than an Open container homogenizing system. This test was run to see how small of a sample can be homogenized using a blade. This test was run to see how the sample homogenized using a blade. This test was run to see how homogenized using a blade without doing
any shaking.

Phycomyces
PRODUCT APPLICATION REPORT
MATERIAL: Phycomyces
VOLUME OF MATERIAL: 1 g
MEDIUM: Chloroform, methanol and hydrochloric acid
VOLUME OF MEDIUM: 10 ml
PROCESS: Homogenization of fibers and shear cells
PREVIOUS OR CURRENT METHOD: Mortar and pestle
EQUIPMENT USED: PRO200 Homogenizer with 7mm generator
APPLICATION AND RESULTS: Worked well, but clogged occasionally.
However, this is not a major problem.

Placenta tissue
PRODUCT APPLICATION REPORT
MATERIAL: Placenta tissue
VOLUME OF MATERIAL: 100 g
MEDIUM: Not reported
VOLUME OF MEDIUM: 2 L
PROCESS: Complete homogenization
PREVIOUS OR CURRENT METHOD: Tekmar Tissue-Mizer
EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300D with 20mm generator
APPLICATION AND RESULTS: Complete homogenization in 15 seconds.

Plant tissue
PRODUCT APPLICATION REPORT
MATERIAL: Plant tissue
VOLUME OF MATERIAL: 50 g
MEDIUM: GTC
VOLUME OF MEDIUM: 100 ml
PROCESS: Homogenization and RNA extraction
PREVIOUS OR CURRENT METHOD: Brinkmannn Polytron
EQUIPMENT USED: PRO200 Homogenizer with 20mm generator
APPLICATION AND RESULTS: Worked nicely.

Plant tissue
PRODUCT APPLICATION REPORT
MATERIAL: Plant tissue
VOLUME OF MATERIAL: 10 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Freeze with liquid nitrogen and break
up with more and pestle
EQUIPMENT USED: PRO200 Homogenizer with 7mm generator
APPLICATION AND RESULTS: Successful homogenization

Popcorn Hot air popped
PRODUCT APPLICATION REPORT
MATERIAL: Popcorn, Hot air popped
VOLUME OF MATERIAL: 400 ml
MEDIUM: None
VOLUME OF MEDIUM: Not reported
PROCESS: Process this material to a level of consistent particle size - ideally this material should pass thru a 40 mesh.
PREVIOUS OR CURRENT METHOD: Unknown
EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300D with 30mm rotor-stator generator The sample was placed in a 473ml open glass container.
APPLICATION AND RESULTS: The sample was placed in a 473m1 glass
container, running at 28,000 rpm. The container was handheld and moved around as well as up and down to allow the generator to cover the total container. The total running time was 45 seconds. While running the sample the top of the container had to be covered to keep the light powder material from being thrown out.

Popcorn Hot air popped
PRODUCT APPLICATION REPORT
MATERIAL: Popcorn, Hot air popped
VOLUME OF MATERIAL: 300 - 400m1
MEDIUM: NA
VOLUME OF MEDIUM: NA
PROCESS: Process this material to a level of consistent particle size - ideally this material should pass thru a 40 mesh.
PREVIOUS OR CURRENT METHOD: Unknown
EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300D with a 2" diameter blade within a 473ml-sealed container.
APPLICATION AND RESULTS: Tests: 1-3 at 300m1 of material. Test: 4
at 400m1 of material.

Test 1: 10,000 rpm - 15/20 seconds, 28,000 rpm - 40/45 seconds on & off.

Test 2: Varied speeds for 5 minutes - on & off.

Test 3: 10,000 rpm - 1 minute, stopped, 20,000 rpm - 1 minute, stopped, 28,000 rpm - 1 minute.

Test 4: 10,000 rpm - 1 minute, stopped, 20,000 rpm - 1 minute, stopped, 28,000 rpm - 1 minute.

RECOMMENDATIONS: This method seemed to work better than using an Open container homogenizing system. There was less mess and the container did not have to be moved around.

Powder
PRODUCT APPLICATION REPORT
MATERIAL: Powder
VOLUME OF MATERIAL: 1 ml
MEDIUM: Aqueous
VOLUME OF MEDIUM: Not Reported
PROCESS: Homogenization of reconstituted polymers
PREVIOUS OR CURRENT METHOD: Hand pestle
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: PRO200 Homogenizer did the job successfully.

Powdered aluminum
PRODUCT APPLICATION REPORT
MATERIAL: Powdered aluminum
VOLUME OF MATERIAL: .5 ml
MEDIUM: None
VOLUME OF MEDIUM: None
PROCESS: Dispersal but no disruption of particle shape or size
PREVIOUS OR CURRENT METHOD: None
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: Good application.

Protein lysates
PRODUCT APPLICATION REPORT
MATERIAL: Protein lysates
VOLUME OF MATERIAL:
MEDIUM: Homogenizing solution containing 10 mM Tris pH 8.0, 
150 mM NaCl, 1 mM EDTA, 1 mM orthovanadate, 
1% Triton X-100,  and 60 mM N-octyl-b-D-glucopyranoside, 
in the presence of protease inhibitors
VOLUME OF MEDIUM:
PROCESS: lysing
PREVIOUS OR CURRENT METHOD: None
EQUIPMENT USED: PRO200 Homogenizer 
APPLICATION AND RESULTS:

At the end of treatment, tumors were fixed in formalin 

and embedded in paraffin for IHC or frozen at −80°C for protein lysates. Protein lysates were obtained homogenizing three times

at high speed at 4°C for 20 minutes. 

Quail liver
PRODUCT APPLICATION REPORT
MATERIAL: Quail liver
VOLUME OF MATERIAL: .05 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Grinding
PREVIOUS OR CURRENT METHOD: Freeze with liquid nitrogen and grind
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: Worked well.

Rat aorta
PRODUCT APPLICATION REPORT
MATERIAL: Rat aorta
VOLUME OF MATERIAL: Not reported
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Break down rat aorta to recover as much RNA as possible
PREVIOUS OR CURRENT METHOD: Brinkmannn polytron after frozen, and
broken with liquid nitrogen.
EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300D with 10mm generator
APPLICATION AND RESULTS: PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer worked successfully and results were better than the previous method.

Rat bowel
PRODUCT APPLICATION REPORT
MATERIAL: Rat bowel
VOLUME OF MATERIAL: 10 g
MEDIUM: TCA
VOLUME OF MEDIUM: Not Reported
PROCESS: Homogenous suspension needed for membrane assays
PREVIOUS OR CURRENT METHOD: Brinkmannn Polytron
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: Worked well and its ease of use were
beneficial in conducting the experiment.

Rat brain
PRODUCT APPLICATION REPORT
MATERIAL: Rat brain
VOLUME OF MATERIAL: 25ml
MEDIUM: PDY buffer
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: PTFE-glass homogenizer
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: Worked well and cut time drastically.

Rat brain liver muscle peripheral nerves
PRODUCT APPLICATION REPORT
MATERIAL: Rat brain, liver, muscle, peripheral nerves
VOLUME OF MATERIAL: 50 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Protein isolation
PREVIOUS OR CURRENT METHOD: Dounce homogenizer
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: Worked very well.

Rat brains
PRODUCT APPLICATION REPORT
MATERIAL: Rat brains
VOLUME OF MATERIAL: 10 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: DNA extraction
PREVIOUS OR CURRENT METHOD: Hand mixer
EQUIPMENT USED: PRO200 Homogenizer with 7mm generator
APPLICATION AND RESULTS: PRO200 Homogenizer achieved desired results and even crushed up the skull that was included in the sample.

Rat epidymous
PRODUCT APPLICATION REPORT
MATERIAL: Rat epidymous
VOLUME OF MATERIAL: 3 ml
MEDIUM: SPW
VOLUME OF MEDIUM: Not reported
PROCESS: Disruption of tissue and release of sperm
PREVIOUS OR CURRENT METHOD: Forceps and scapel
EQUIPMENT USED: PRO200 Homogenizer with 7mm & 10mm generator
APPLICATION AND RESULTS: 7mm too small but best results were
attained with 10mm generator. Total disruption was achieved.

Rat jaw and teeth
PRODUCT APPLICATION REPORT
MATERIAL: Rat jaw and teeth
VOLUME OF MATERIAL: .05 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Mortar and pestle
EQUIPMENT USED: PRO200 Homogenizer with 7mm generator
APPLICATION AND RESULTS: Completely homogenized sample.

Rat leg muscles
PRODUCT APPLICATION REPORT
MATERIAL: Rat leg muscles
VOLUME OF MATERIAL: 5 ml (7mm gen.) and 20 ml (10mm gen.)
MEDIUM: BSA
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Mortar and pestle
EQUIPMENT USED: PRO200 Homogenizer with 7mm & 10mm generator
APPLICATION AND RESULTS: Ran very well. Saw teeth on 7mm and 10mm
were perfect in homogenizing the sample.
Rat Liver
PRODUCT APPLICATION REPORT
MATERIAL: Rat Liver
VOLUME OF MATERIAL: 150 grams of tissue
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Brinkmannn Polytron PT10 / 35
EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300D with 30m generator
APPLICATION AND RESULTS: Successfully homogenized the rat liver.

Rat spinal cord and root ganglia
PRODUCT APPLICATION REPORT
MATERIAL: Rat spinal cord and root ganglia
VOLUME OF MATERIAL: .5 ml
MEDIUM: Lithium chloride
VOLUME OF MEDIUM: 1 ml
PROCESS: Complete homogenization
PREVIOUS OR CURRENT METHOD: Mortar and Pestle
EQUIPMENT USED: PRO200 Homogenizer with 7mm generator
APPLICATION AND RESULTS: PRO200 worked well on the sample.

Rat tongue and tails
PRODUCT APPLICATION REPORT
MATERIAL: Rat tongue and tails
VOLUME OF MATERIAL: 1 ml
MEDIUM: ECTA, DDT
VOLUME OF MEDIUM: 1.5 ml
PROCESS: Isolate DNA protein
PREVIOUS OR CURRENT METHOD: Grind in blender and Mortar and pestle
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: Successfully isolated the DNA protein
from the tongue and tails.

Rat uterii
PRODUCT APPLICATION REPORT
MATERIAL: Rat uteri
VOLUME OF MATERIAL: 50 ml
MEDIUM: Water
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Mortar and pestle, Brinkmannn Polytron
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: Did a better job than the Polytron, and
it produced less noise.

Raw sewage
PRODUCT APPLICATION REPORT
MATERIAL: Raw sewage
VOLUME OF MATERIAL: 5ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Analysis of raw sewage
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer with 7mm generator
APPLICATION AND RESULTS: Works well

Retina
PRODUCT APPLICATION REPORT
MATERIAL: Retina
VOLUME OF MATERIAL: Retina was cut into pieces
MEDIUM: Homogenization buffer
VOLUME OF MEDIUM: 200 ul
PROCESS: Not reported
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: Successfully homogenized

Rice and barley seeds
PRODUCT APPLICATION REPORT
MATERIAL: Rice and barley seeds
VOLUME OF MATERIAL: 10 ml (both 7mm and 10mm gen. Test)
MEDIUM: Organic solvents
VOLUME OF MEDIUM: Not Reported
PROCESS: Complete dispersal of starch material
PREVIOUS OR CURRENT METHOD: Liquid nitrogen then mortar and pestle
EQUIPMENT USED: PRO200 Homogenizer with 7mm & 10mm generator
APPLICATION AND RESULTS: Best results with 10mm saw tooth.

Rice seeds (grain)
PRODUCT APPLICATION REPORT
MATERIAL: Rice seeds (grain)
VOLUME OF MATERIAL: 5:1 ratio to water
MEDIUM: Water
VOLUME OF MEDIUM: Not reported
PROCESS: To get out the protein
PREVIOUS OR CURRENT METHOD: Unknown
EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300D with 20m generator
APPLICATION AND RESULTS: Experienced excessive denaturing from air
bubbles.
RECOMMENDATIONS: Air bubbles were caused by not having the generator deep enough within the medium. It is recommended that either a smaller generator be used or the volume of medium is increased.

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Rodent embryo