What I'm Homogenizing S-Z

Salmon sperm

PRODUCT APPLICATION REPORT

MATERIAL: Salmon sperm VOLUME OF MATERIAL: 5ml (7mm gen.) and 25 ml (20mm gen.) MEDIUM: Blocking solution VOLUME OF MEDIUM: Not reported PROCESS: Liquefaction of sperm for DNA analysis PREVIOUS OR CURRENT METHOD: 23-guage-liquefaction needle EQUIPMENT USED: PRO200 Homogenizer with 7mm & 10mm generator APPLICATION AND RESULTS: Desired liquefaction results in 2-3 minutes.

Sea Weed

PRODUCT APPLICATION REPORT

MATERIAL: Sea Weed VOLUME OF MATERIAL: 20 ml MEDIUM: Not Reported VOLUME OF MEDIUM: Not Reported PROCESS: Homogenization PREVIOUS OR CURRENT METHOD: Polytron PT1200 (did not homogenize sea weed - material wrapped around generator) EQUIPMENT USED: PRO200 Homogenizer with 10mm generator APPLICATION AND RESULTS: PRO200 Homogenizer with l0mm generator worked successfully. It out performed PT1200

Silica

PRODUCT APPLICATION REPORT

MATERIAL: Silica gel VOLUME OF MATERIAL: 400 ml MEDIUM: Aqueous VOLUME OF MEDIUM: Not reported PROCESS: Dispersion PREVIOUS OR CURRENT METHOD: Blender EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300D with 20m generator APPLICATION AND RESULTS: successful.

Sediment PRODUCT APPLICATION REPORT MATERIAL: Sediment VOLUME OF MATERIAL: Not reported MEDIUM: Sediment water VOLUME OF MEDIUM: Not reported PROCESS: Not reported PREVIOUS OR CURRENT METHOD: Not reported EQUIPMENT USED: PRO250 Homogenizer APPLICATION AND RESULTS: Successfully homogenized Link DOI: http://dx.doi.org/10.1021/es203528v Silicone

PRODUCT APPLICATION REPORT

MATERIAL: Silicone VOLUME OF MATERIAL: 5 ml MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: Not reported PREVIOUS OR CURRENT METHOD: Not reported EQUIPMENT USED: PRO200 Homogenizer with 7mm generator APPLICATION AND RESULTS: The PRO200 Homogenizer with 7mm generator produced excellent results.

Skeletal muscle (human)

PRODUCT APPLICATION REPORT

MATERIAL: Skeletal muscle (human)  VOLUME OF MATERIAL: not reported MEDIUM: lysis buffer VOLUME OF MEDIUM: Not reported PROCESS: "Homogenized for 20 strokes" PREVIOUS OR CURRENT METHOD: Not reported EQUIPMENT USED: PRO200 Homogenizer APPLICATION AND RESULTS: Excellent results. Link DOI: http://dx.doi.org/10.1016/j.jprot.2012.03.024

Skeletal muscle fat and liver powder

PRODUCT APPLICATION REPORT

MATERIAL: Skeletal muscle, fat, and liver powder VOLUME OF MATERIAL: 1 g MEDIUM: GTC buffer or phosphate buffer VOLUME OF MEDIUM: 10 ml PROCESS: RNA extraction through homogenized suspension PREVIOUS OR CURRENT METHOD: Brinkmannn Polytron EQUIPMENT USED: PRO200 Homogenizer with 7mm generator APPLICATION AND RESULTS: Worked very well and saved time and effort.

Snails

PRODUCT APPLICATION REPORT

MATERIAL: Snails VOLUME OF MATERIAL: 5 ml MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: Homogenization PREVIOUS OR CURRENT METHOD: Not reported EQUIPMENT USED: PRO200 Homogenizer with 7mm generator APPLICATION AND RESULTS: PRO200 Homogenizer with 7mm saw tooth generator was able to successfully homogenize the mini snails and their shells completely.

Skin fibroblasts

PRODUCT APPLICATION REPORT

MATERIAL: Skin fibroblasts VOLUME OF MATERIAL: 5 ml (5mm gen.) 50 ml (10mm gen.) MEDIUM: Salt buffer VOLUME OF MEDIUM: Not reported PROCESS: Complete homogenization PREVIOUS OR CURRENT METHOD: None EQUIPMENT USED: PRO200 Homogenizer with 5mm & 10mm generator APPLICATION AND RESULTS: Achieved desired results successfully

Soil

PRODUCT APPLICATION REPORT

MATERIAL: Soil VOLUME OF MATERIAL: 200 ml MEDIUM: None VOLUME OF MEDIUM: None PROCESS: Homogenization of dry soil PREVIOUS OR CURRENT METHOD: Meat grinder EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300 D homogenizer withblade and sealed chamber assembly APPLICATION AND RESULTS: Sample ground up into fine powder

Soybean cotyledon

PRODUCT APPLICATION REPORT

MATERIAL: Soybean cotyledon VOLUME OF MATERIAL: 100 ml MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: Grinding PREVIOUS OR CURRENT METHOD: Blender EQUIPMENT USED: PRO200 Homogenizer with 10mm generator APPLICATION AND RESULTS: Worked well.

Soybean cotyledons

PRODUCT APPLICATION REPORT

MATERIAL: Soybean cotyledons VOLUME OF MATERIAL: 50 ml MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: Homogenization PREVIOUS OR CURRENT METHOD: Mortar and pestle EQUIPMENT USED: PRO200 Homogenizer with 10mm generator APPLICATION AND RESULTS: Complete homogenization

Soybean plant - various parts

PRODUCT APPLICATION REPORT

MATERIAL: Soybean plant VOLUME OF MATERIAL: 5 ml MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: Rupture of samples PREVIOUS OR CURRENT METHOD: Mortar and pestle EQUIPMENT USED: PRO200 Homogenizer with 7mm & 10mm generator APPLICATION AND RESULTS: Successful application

Spleen

PRODUCT APPLICATION REPORT

MATERIAL: Spleen VOLUME OF MATERIAL: 10 ml MEDIUM: Buffer VOLUME OF MEDIUM: 40 ml PROCESS: Spleen disruption PREVIOUS OR CURRENT METHOD: Tekmar Tissue-Mizer EQUIPMENT USED: PRO200 Homogenizer with 10mm generator APPLICATION AND RESULTS: Good results.

Spore strips

PRODUCT APPLICATION REPORT

MATERIAL: Spore strips VOLUME OF MATERIAL: 1 strip MEDIUM: Tryptic Soy broth VOLUME OF MEDIUM: 20 ml PROCESS: Break up to recover bacteria PREVIOUS OR CURRENT METHOD: Mortar and pestle and vortex stirrer EQUIPMENT USED: PRO200 Homogenizer with 10mm generator APPLICATION AND RESULTS: Broken up easily. Increased cell disruption achieve through longer homogenization.

Sturgeon barbles

PRODUCT APPLICATION REPORT

MATERIAL: Sturgeon barbles VOLUME OF MATERIAL: 4 ml MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: Mitochondria extraction PREVIOUS OR CURRENT METHOD: Mortar and pestle EQUIPMENT USED: PRO200 Homogenizer with 7mm generator APPLICATION AND RESULTS: 7mm saw tooth achieved desired results in five minutes. To make sure that generated heat would not harm sample, used ice bath.

Ticks

PRODUCT APPLICATION REPORT

MATERIAL: Ticks VOLUME OF MATERIAL: 2 ticks MEDIUM: PBS/PRS VOLUME OF MEDIUM: 50 ml PROCESS: Obtain 100% homogenization of the ticks. PREVIOUS OR CURRENT METHOD: Manual homogenization EQUIPMENT USED: PRO200 Homogenizer with 5mm generator APPLICATION AND RESULTS: Homogenized ticks without any problems. System worked very well.

Tibialis anterior muscle

PRODUCT APPLICATION REPORT

MATERIAL: Tibialis anterior muscle VOLUME OF MATERIAL: 20 mg MEDIUM: potassium phosphate buffer (50 mM K 2 HPO 4 /KH 2 PO 4 , 1 mM EDTA, 0.1 mM DTT, pH 7.4) supplemented with protease inhibitors VOLUME OF MEDIUM: PROCESS: Homogenized PREVIOUS OR CURRENT METHOD: EQUIPMENT USED: PRO Homogenizer  APPLICATION AND RESULTS: DOI: http://dx.doi.org/10.1371/journal.pone.0081879

Tissue

PRODUCT APPLICATION REPORT

MATERIAL: Tissue VOLUME OF MATERIAL: .25 ml MEDIUM: GTI VOLUME OF MEDIUM: .5 ml PROCESS: Homogenize tissue to isolate RNA PREVIOUS OR CURRENT METHOD: Brinkmannn instruments EQUIPMENT USED: PRO200 Homogenizer with 7mm generator APPLICATION AND RESULTS: Results were extremely successful

Tissue

PRODUCT APPLICATION REPORT

MATERIAL: Tissue VOLUME OF MATERIAL: 10 ml MEDIUM: Not reported VOLUME OF MEDIUM: 25 ml PROCESS: Homogenize tissue with no degradation of RNA PREVIOUS OR CURRENT METHOD: Not reported EQUIPMENT USED: PRO200 Homogenizer with 10mm generator APPLICATION AND RESULTS: Excellent results

Tumor

PRODUCT APPLICATION REPORT

MATERIAL: Tumor VOLUME OF MATERIAL: Not reported MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: Not reported PREVIOUS OR CURRENT METHOD: Break down tumor without disrupting cells EQUIPMENT USED: PRO200 Homogenizer with 7mm generator APPLICATION AND RESULTS: Successfully broke down the tumor without disrupting cells.

Vanilla Callus tissue culture

PRODUCT APPLICATION REPORT

MATERIAL: Vanilla Callus tissue culture VOLUME OF MATERIAL: 1/8"-1/4" MEDIUM: Water VOLUME OF MEDIUM: 2-10 ml PROCESS: Complete homogenization PREVIOUS OR CURRENT METHOD: Mortar and pestle EQUIPMENT USED: PRO200 Homogenizer with 7mm generator APPLICATION AND RESULTS: Successfully broke the sample down with the 7mm generator.

Various creams

PRODUCT APPLICATION REPORT

MATERIAL: Various creams VOLUME OF MATERIAL: 100-250 ml MEDIUM: None VOLUME OF MEDIUM: 250-500 ml PROCESS: Emulsion with even disbursement PREVIOUS OR CURRENT METHOD: Brinkmannn, IKA, and other various mixers EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300 D homogenizer with blade and sealed chamber assembly APPLICATION AND RESULTS: Complete emulsification and disbursement with no lumps through blade system. Replaces other methods as best application.

Various Eye Parts

PRODUCT APPLICATION REPORT

MATERIAL: Various eye parts VOLUME OF MATERIAL: 5 ml MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: Break down tissue to extract cells PREVIOUS OR CURRENT METHOD: None EQUIPMENT USED: PRO200 Homogenizer with 7mm generator APPLICATION AND RESULTS: Worked well

Virus

PRODUCT APPLICATION REPORT

MATERIAL: Virus VOLUME OF MATERIAL: 25 ml MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: Extraction of virus for electrophoresis assay PREVIOUS OR CURRENT METHOD: Hand turned mixer EQUIPMENT USED: PRO200 Homogenizer with 10mm generator APPLICATION AND RESULTS: Excellent homogenization of sample.

Waste bacteria

PRODUCT APPLICATION REPORT

MATERIAL: Waste bacteria VOLUME OF MATERIAL: .05 ml (5mm gen.) and 50 ml (10mm gen.) MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: Break down of bacteria through homogenization PREVIOUS OR CURRENT METHOD: Not reported EQUIPMENT USED: PRO200 Homogenizer with 5mm & 10mm generator APPLICATION AND RESULTS: Products proved to be successful

Water oil mix

PRODUCT APPLICATION REPORT

MATERIAL: Water oil mix (olive oil) VOLUME OF MATERIAL: MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: PREVIOUS OR CURRENT METHOD: Not reported EQUIPMENT USED: PRO200 Homogenizer  APPLICATION AND RESULTS: DOI: Products proved to be successful http://dx/doi.org/10.1039/C3FO60380F

Wheat flour

PRODUCT APPLICATION REPORT

MATERIAL: Wheat flour VOLUME OF MATERIAL: water flour ratio 1.7 MEDIUM: water VOLUME OF MEDIUM: water flour ratio 1.7 PROCESS: 6000 rpm, 2 minutes PREVIOUS OR CURRENT METHOD: Not reported EQUIPMENT USED: PRO350PC Homogenizer with 37mm PRO Quick Connect Generator APPLICATION AND RESULTS: Successfully homogenized and standard was established for bench-scale high-shear wet-milling test Link DOI: http://dx.doi.org/10.1016/j.jfoodeng.2012.02.018

Wheat leaves

PRODUCT APPLICATION REPORT

MATERIAL: Wheat leaves VOLUME OF MATERIAL: 25 ml MEDIUM: Extraction buffer VOLUME OF MEDIUM: 25 ml PROCESS: Fine grinding of plant tissue for DNA analysis PREVIOUS OR CURRENT METHOD: Mortar and pestle after freezing with liquid nitrogen EQUIPMENT USED: PRO200 Homogenizer with 10mm generator APPLICATION AND RESULTS: DNA was broken down into short pieces that could not analyze. Big chunks were needed for proper examination. RECOMMENDATIONS: Perhaps a blade system can give the larger sample sizes that are needed for this type of application.

Whole cornea

PRODUCT APPLICATION REPORT

MATERIAL: Whole cornea VOLUME OF MATERIAL: 5 ml MEDIUM: Not reported VOLUME OF MEDIUM: Not reported PROCESS: Homogenization PREVIOUS OR CURRENT METHOD: Mortar and pestle EQUIPMENT USED: PRO200 Homogenizer with 7mm & 10mm generator APPLICATION AND RESULTS: The 7mm generator was too small to handle large cornea chunks. However, the 10mm was perfect for the application.

Wild tomato vines

PRODUCT APPLICATION REPORT

MATERIAL: Wild tomato vines VOLUME OF MATERIAL: 3g (5mm gen.) and 6 g (7mm gen.) MEDIUM: Aqueous buffer VOLUME OF MEDIUM: 5 ml (5mm gen.) and 10 ml (7mm gen.) PROCESS: Dispersion and homogenization PREVIOUS OR CURRENT METHOD: Virtishear mixer EQUIPMENT USED: PRO200 Homogenizer with 5mm & 7mm generator APPLICATION AND RESULTS: Worked excellent.

Yeast cells

PRODUCT APPLICATION REPORT

MATERIAL: Yeast cells VOLUME OF MATERIAL: 2 ml MEDIUM: Water VOLUME OF MEDIUM: 2 ml PROCESS: Break down yeast cells PREVIOUS OR CURRENT METHOD: Glass beads and Braun system EQUIPMENT USED: PRO200 Homogenizer with 7mm generator APPLICATION AND RESULTS: Tried using a 7mm generator with glass beads added. The beads were ground up but the yeast cells were not broken up enough. A 10mm generator will probably break the yeast cells apart enough.

Zebrafish (embryo-larval)

PRODUCT APPLICATION REPORT

MATERIAL: Zebrafish (embryo-larval) VOLUME OF MATERIAL: MEDIUM: Trizol reagent VOLUME OF MEDIUM: PROCESS: Isolation of RNA PREVIOUS OR CURRENT METHOD: EQUIPMENT USED: PRO200 Homogenizer APPLICATION AND RESULTS: DOI: Thirty embryos per group were homogenized with a Bio-Gen PRO200 homogenizer for the isolation of total RNA, using Trizol reagent. http://dx.doi.org/10.1016/j.chemosphere.2013.10.014

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